中文摘要：
目前學界對大腦受損血管修復過程中的細胞調控機制尚不明確，針對糖尿病等存在血管病變高危因素人群的相關研究尤為匱乏。本研究剖析了腦組織固有小膠質細胞與浸潤性巨噬細胞在破損腦微血管修復進程中的作用。
研究結合活體延時成像、基因表達分析及免疫組化技術，鑒定出一類獨特的半乳糖凝集素 3（Gal3）陽性吞噬型巨噬細胞；該細胞群與固有小膠質細胞存在明顯區別，會浸潤并聚集于糖尿病小鼠的血管損傷部位，且與微血管清除過程密切相關。在糖尿病小鼠體內使用荷蘭Liposoma氯膦酸鹽脂質體清除這類浸潤性巨噬細胞后，細胞吞噬活性顯著下降，同時能夠抑制損傷后微血管的丟失。
上述研究結果揭示了浸潤性 Gal3 陽性巨噬細胞在腦損傷后介導血管清除的全新作用，也為后續開展相關研究提供依據：明確清除該類細胞是否能夠促進高危人群的腦血管修復。
英文摘要：
The cellular events that dictate the repair of damaged vessels in the brain, especially in those with vascular risk factors such as diabetes, is poorly understood. Here, we dissected the role of resident microglia and infiltrative macrophages in determining the repair of ruptured cerebral microvessels. Using in vivo time-lapse imaging, gene expression analysis, and immunohistochemistry, we identified a unique population of phagocytic Galectin 3 (Gal3) expressing macrophages, distinct from resident microglia, which infiltrated and aggregated at the site of injury in diabetic mice and were associated with the elimination of microvessels. Depletion of these infiltrative macrophages in diabetic mice attenuated phagocytic activity and prevented the loss of blood vessels after injury. These findings highlight a previously unknown role for infiltrative Gal3 expressing macrophages in promoting vessel elimination after brain injury and provide impetus for future studies to determine whether depleting these cells can facilitate vascular repair in at risk populations.
論文信息：
論文題目：Invasion of phagocytic Galectin 3 expressing macrophages in the diabetic brain disrupts vascular repair
期刊名稱：Science Advances
時間期卷：Vol 7, Issue34(2021)
在線時間：2021年8月18日
DOI: 10.1126/sciadv.abg2712
產品信息：
貨號：CP-005-005
規格：5ml+5ml
品牌：Liposoma
產地：荷蘭
名稱：Clodronate Liposomes&Control Liposomes
辦事處：靶點科技
Clodronate Liposomes氯膦酸鹽脂質體靜脈注射，清除外周巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于Science Advances：吞噬型半乳糖凝集素 3 陽性巨噬細胞浸潤糖尿病小鼠腦組織會阻礙血管修復。
Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體清除巨噬細胞的材料和方法：
In vivo macrophage depletion
For drug intervention experiments, mice received intravenous CLR (50 mg/kg; Liposoma B.V.) 2 days before CMB induction, on the day of CMB induction, and 2 days later. This treatment regimen ensured sustained depletion of peripheral macrophages. Diff-Quik histological staining confirmed depletion of circulating immune cells in treated animals.
藥物干預實驗中，于腦微出血造模前 2 天、造模當日及造模后 2 天，對小鼠尾靜脈注射氯膦酸二鈉脂質體（CLR，給藥劑量 50 mg/kg，廠商：Liposoma B.V.）。該給藥方案可實現外周巨噬細胞的持續性清除。經迪夫快速（Diff-Quik）組織染色驗證，給藥小鼠外周循環免疫細胞已被有效清除。
巨噬細胞清除材料和方法文獻截圖：吞噬型半乳糖凝集素 3 陽性巨噬細胞浸潤糖尿病小鼠腦組織會阻礙血管修復
